Laboratory Testing
BS EN 13623:2020

Legionella Efficacy Testing Results

Independent UKAS-accredited laboratory testing confirms ChloroKlean Plus meets the requirements of BS EN 13623:2020 for bactericidal activity against Legionella pneumophila.

Test Conclusion

UKAS Accredited Laboratory

ChloroKlean Plus at concentrations of 83ppm, 60ppm and 50ppm met the log reduction requirements as specified in EN 13623 (4 lg) with a contact time of 60 minutes for Legionella pneumophila NCTC 11192.

Test Conditions

Test Standard

BS EN 13623:2020

Test Temperature

30°C

Contact Time

60 minutes

Test Organism

Legionella pneumophila NCTC 11192

Additional Test Parameters

  • Interfering Substance: Yeast Extract (0.0005% final concentration)
  • Diluent: Buffered ferrous hard water
  • Neutralisation Method: Membrane Filtration using Rinsing Diluent with Sodium Thiosulphate
  • Incubation Temperature: 36°C

Results by Concentration

The standard requires a minimum 4 lg (log) reduction in bacterial count to pass. Results at different ChloroKlean Plus concentrations:

83 ppm
Log reduction: >4 lg
Pass
60 ppm
Log reduction: >4 lg
Pass
50 ppm
Log reduction: >4 lg
Pass
40 ppm
Log reduction: <4 lg
Partial

About BS EN 13623:2020

BS EN 13623:2020 is the European standard for evaluating the bactericidal activity of chemical disinfectants against Legionella in aqueous systems. It is a Phase 2, Step 1 quantitative suspension test that establishes the baseline efficacy of products under controlled laboratory conditions.

Test Purpose

Evaluates whether a disinfectant can achieve the required 4 log reduction against Legionella pneumophila under standardised conditions relevant to cooling water systems.

Regulatory Relevance

EN 13623 testing supports BPR product authorisation claims and provides evidence of efficacy for HSE compliance under HSG274 and ACOP L8 guidance.

Key Takeaway

ChloroKlean Plus demonstrates proven efficacy against Legionella pneumophila at concentrations as low as 50ppm with a 60-minute contact time, meeting the stringent requirements of BS EN 13623:2020 for cooling water treatment applications.